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This article investigates the syntactic parameters of the agreement/case system in three northwest Iranian languages, including Tati, Talshi, and Vafsi. Baker (2008) shows that the close relationship between agreement and case that Chomsky (2000) formalized it as the Operation Agree is a parametric matter and in all languages, the agreement does not depend on the structural case of the noun phrase. In this regard, he considers the existence of two parameters:  parameter (1) Direction of agreement parameter (DAP) which is based on the c-commanding condition and The Case Dependency of Agreement Parameter (CDAP) or Operation Agree and he believes that the Operation Agree or parameter (2) is only one the agreement parameters in universal grammar, not a principle ones. Also, in a language, all functional heads, if they agree with a noun phrase, are homogeneously subject to the fixed agreement parameter in that language. In this regard, we evaluate how the two parameters of Direction of agreement parameter (DAP) and The Case Dependency of Agreement Parameter (CDAP) are fixed in the functional head (T) and moreover we deal with other functional heads including determiners (D), prepositions (P) and auxiliary verbs (AUX). The results show that in these three Iranian languages, the functional head (T) is subject to the CDAP parameter, not the DAP one and the c-commanding condition is not necessary for agreement. Moreover, in par with the functional head (T), other functional heads are homogenously subject to CDAP. The method of data collection was the library method and interview.

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Among the lexical units of a language, all of which can be subjected to semantic expansion according to selection and combination, verbs are quite susceptible to radial and semantic expansion due to their nature. This article tries to use the cognitive approach to investigate the semantic expansion of the simple triad form of the event "Zarb" in the Arabic language. And in a descriptive-analytical way, while enumerating the marginal expansions of this verb, explain how they are produced and also the semantic sharing relationship between the derivatives of the same root. About 80 usages were extracted from the most advanced to the latest Arabic dictionaries for the event "Zarb", which, excluding similar usages, resulted in 32 radial meanings for this event. The result showed that the existence of a semantic relationship between the derivatives of a word can be confirmed in the light of the derivational property of the Arabic language. Therefore, from the most unmarked meaning of "stroke" to its metaphorical expansions, each of them conveys the concept of "pressure and force" in a way. Therefore, the claim that the meaning of "beating" is late in this event is questionable. Also, most of the marginal expansions of the verb "to multiply" are the product of the change in the most frequently used elements accompanying this event, i.e. space scenes. In addition, in the virtual expansion of the event "hit", the necessary-necessary interest and the cause-cause have more frequency in producing the radial meanings of this event.
 

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Khalaj language is an endangered and independent branch of Turkic languages and is spoken in some villages in the center of Iran. According to researchers, the study of Khalaj language is very important because it preserves the linguistic features of Proto-Turkic. The present study provides an overview of encoding two grammatical case markers: comitative and instrumental, in Khalaj language based on typological approaches. The corpus of study consists of available data in Iran's linguistic atlas (ILA) which have been gathered based on the questionnaire prepared for this atlas. The data belongs to 16 villages in Qom province and 4 villages in Markazi province. The corpus of research includes 100 sentences which are analyzed based on the theoretical framework. According to the analyzed data, it is concluded that there is the same marker for encoding the different cases, i.e., comitative and Instrumental. Khalaj language employs the identity strategy for encoding this marker which is a postposition and attaches to the head of noun phrases.

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The aim of this research was to study the effects of whey protein concentrate (WPC) and casein hydrolysate (CH) on chemical (pH and titratable acidity), physical (consistency and syneresis) and sensory properties of yogurt containing probiotic bacteria. Reconstituted skim milk at 10% total solids was fortified with 1 and 2% of WPC, CH and blend of them (WPC to CH ratios of 1:1). Reconstituted skim milks were made with 11 and 12% total solids as control. Bioyogurts were prepared with commercial probiotic starter culture (Lactobacillus acidophilus, Bifidobacterium sp., Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus). Fermentation was stopped at pH=4.6 and samples stored at 4°C for 21 days. Addition of WPC resulted in the highest pH value and the lowest titratable acidity. Acidity values of supplemented bioyogurt samples increased between 0.12 - 0.19% during 21 days of storage. Mَoreovere syneresis of these yogurts was lower and their consistency was higher than control. Bioyogurts containing WPC had the lowest syneresis and samples containing CH or WPC had the highest consistency. With regards to total acceptability, the best samples were bioyogurts supplemented with WPC and blend of WPC and CH.

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The aim of this research was to study the effects of whey protein concentrate (WPC) and casein hydrolysate (CH) on chemical (pH and titratable acidity), physical (consistency and syneresis) and sensory properties of yogurt. Reconstituted skim milk at 10% total solids was fortified with 1 and 2% of WPC, CH and blend of them (WPC to CH ratios of 1:1). For comparison, reconstituted skim milks were made with 11 and 12% total solids as control. Yogurts were prepared with commercial starter culture (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and their fermentation proccess were stopped at pH=4.6 and samples stored at 4°C up to 21 days. The lowest pH value was obtained when milk base was supplemented with WPC. pH values of all yogurt samples did not change significantly during 21 days of storage and ~0.18% increase in titratable acidity was observed during storage period. The lowest syneresis was observed in yogurts containing blend of WPC and CH. Moreover, the syneresis values of these yogurts increased slower than other samples during storage. Consistency of fortified yogurts was higher than control yogurt and the consistency values of these samples reduced slower than control sample during 21 days of storage. With regards to total acceptability, the best samples were yogurts supplemented with blend of WPC and CH.

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The current Internet inherently has a degree of survivability due to the connection less IP Protocol. Dynamic routing Protocols are designed to react to faults by changing routes when routers learn about topology changes via routing information updates (e.g., link status advertisements). Loss of Quality of service (QoS) has not been an issue because current Internet traffic is the best-effort. On the contrary, the multi protocol label switching (MPLS) approach is connection-oriented, which implies greater potential vulnerability to faults. At the same time, MPLS will support integrated services, which are more sensitive to loss of service. Reliability is becoming more important as more users depend on the internet for critical communication services and expect a higher level of performance. Usually, fault recovery is attempted first at the lowest layer, and escalated to the next layer if recovery was unsuccessful or impossible. Fault recovery capabilities in the MPLS layer are needed as well to decouple MPLS from dependence on physical layer fault recovery mechanisms which may differ between networks. This paper proposes an enhanced-scheme for fast rerouting to pre-assigned label-switched paths (LSPs) in case of LSP or link failures. In order to minimize backup resources, it allows possibility of splitting traffic of faulty LSP onto available alternative LSPs for fault recovery. We use Pre-assigned backup LSPs for restoration, when fault occurs. Total traffic throughput and resource utilization can be maximized if the traffic of faulty LSP is split over multiple pre-assigned LSPs. In this paper a new approach to providing fault tolerance in MPLS networks using case-based reasoning (CBR) as a method to find out the amount of traffic forwarded on each pre-assigned LSP based on past experiences of loading process is presented. The pre-assigned LSPs and the percentage of traffic splitting are calculated off-line based on desired QoS and capacity constraints. Also we evaluate the operation of successful decomposition of traffic based on the two mentioned constraints by using CBR, when the number of backup LSPs as a complexity factor increase. In another point of view, in cases when there is no possibility of using the experiences successfully, there would be no other way than using the erroneous unsuccessful experiences. We thus solved our recovery problem by using, first incorrect databases in our experiments, to moving later towards decreasing the error rate in a gradual manner.

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The present paper focuses on the effects of discourse and pragmatic factors on the determination of case system in Hawrami. Du Bois (1987) argues that dominant case systems in any language cannot be thoroughly accounted for unless pragmatic factors are taken into consideration. He further shows that certain features of narrative discourse creates a special discourse tendency, called “Preferred Argument Structure”, whose defining characteristics are exactly the same as those of “Ergative systems”. The way this pragmatic tendency competes with other pragmatic factors, which exhibit accusative-like features, ultimately determines the case system in syntax. In this paper, it is first shown that, although preferred argument structure is observed in all syntactic constructions of Hawrami, its effect in aorist constructions is more prominent than in the present constructions, and this very fact causes the former set of constructions to become Ergative. Other competing pragmatic tendencies like topic animacy and topic continuity, unlike the preferred argument structure, always motivate the accusative case pattern in syntax. The effects of each of these two factors on determination of accusative case system in the present constructions are not the same; only topic animacy has the potential to set the ultimate case system in syntax as accusative. From a theoretical point of view, the empirical findings of this research may lend more support to those functionally-oriented theories of language, which consider the syntactic properties of languages as direct results of pragmatic considerations and communicative needs of speakers.   

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Laccase enzymes are polyphenol oxidase that catalyze the oxidation of wide range of phenolic components including phenols, polyphenols, aromatic amines and non-phenolic substitution with molecular oxygen as electron acceptor. So these enzymes have biotechnological application such as wastewater treatment system, bioremediation of soil pollution and etc. Result from previous studies showed an increase in thermal stability of bacterial laccase from Bacillus sp. HR03 using site directed mutagenesis and the effect of E188 residue on the surface regions at the interface between domain 1 and 2 in stability was confirmed. The aim of the present work was to investigate the effect of this amino acid substitution on enzyme activity in the presence of dimethyl sulfoxide and dimethylformamide as organic solvents. Compression of kinetic parameters including Kcat / Km ، ∆∆G‡, C50 showed significant increases in the mutant enzyme than wild type enzyme, that industrial application of the enzyme will be easy.

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Laccase (benzenediol: oxygen oxidoreductase), is a multicopper polyphenol oxidase enzyme which has glycoprotein structure. The Researches are indicated that laccase enzyme can play role in detoxification of aromatic pollutants (as petroleum derivatives) and conversion them to less toxic compounds. On the other hand, because of its extensive, fibrous root system; Festuca arundinacea, creates an appropriate environment that causes increased catalysis of petroleum contaminants. Considering the fact that increasing in catalysis of pollutants can be provided by presence and changes in activity of various plant enzymes, in this research; the changes in laccase activity of Festuca's vegetative organs under soil pollution with different concentration of diesel fuel has been investigated. For this purpose, at first, the seeds of Festuca were cultivated in pots containing diesel fuel polluted soils and also control pots in greenhouse conditions. Then in specified time treatments, plants were harvested and plant extract containing laccase were extracted from aerial parts and roots of the plant, separately. After doing centrifuge, changes in enzyme activity were calculated by spectrophotometer. The results show that creation of soil pollution treatments compared with control samples, leads to increase in laccase activity in many cases. In other words, by increasing in laccase activity, the plant will increase its potency of decomposition and assimilation of pollutant hydrocarbons.

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Tâti is one of the languages, which besides applying other means such as agreement and word order, uses case marking of dependents to show the grammatical relations in a sentence. Tâti is among the North-west Iranian languages. Tâti dialects are dispersed in some cities and villages in Ardebil, Qazvin, Zanjan and Gilan provinces. The Tâti variety studied in this article is the Dəravi variety, which is spoken in Khalkhâl. This article intends to study the case system of Dəravi.  Although this variety of Tâti has retained some ancient characteristics, its case system has not been studied yet. This variety is highly endangered as its young speakers immigrate to neighboring cities in search of work, and they no longer transmit their mother language to the next generations.   

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  The aim of this study was to demonstrate the inhibitory capacity of two strains of  gram positive bacilli, isolated from intestinal content of Persian sturgeon, against Listeria monocytogenes growth. Two strains Lactobacillus casei AP 8 and Lactobacillus plantarum A P 12 , were screened for their antilisterial activity against.  L. monocytogenes, using a disk diffusion agar test. However, L. casei AP 8 always had the highest inhibitory effect. The spoiling potential and antilisterial capacity of bacterial strains was tested in sterile cold smoked roach (CSR) blocks inoculated with 10⁴ CFU g^− 1 of lactic bacteria and 10² CFU g^-1 of Listeria monocytogenes and then stored for 10 days at 4 °C followed by 30 days at 20 °C. L. casei AP8 grew a little faster L. plantarum A P 12 and none of them showed any adverse effect on quality of the product ( i.e. no total volatile basic nitrogen (TVBN) production and no acidification. Lactobacillus casei AP8 was the most efficient strain, maintaining the level of L. monocytogenes at <50 CFU/ g  during  40 dayss of storage at 4 and 20°C. In conclusion, biopreservation of cold smoked roach using bacterial cultures such as L. casei AP8  is a promising way to inhibit the growth of pathogenic bacteria such as L. monocytogenes with low effect on the product quality.

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Adjacency condition is  one of the syntactic constraints on case assignment in many languages such as English, Dutch, Turkish and Hindi. Many linguists studied adjacency condition in some languages and some of them including  Chomsky (1981) and  Stowell (1981) confirmed the role of  this condition in generative grammar and some linguists including Johnson (1991) rejected it in generative grammar. Opponents of this hypothesis believe that adjacency condition can be replaced by projection principle and binary branching condition. In this research, we try to investigate the role of adjacency condition in Persian. Data analysis indicates that assigning structural case to subject by inflection head and also assigning structural case to definite direct object by verb are not subject to adjacency condition. In fact, the position of definite direct object and inflection head is in the specifier of inner verb phrase core and at the end of sentence, respectively which they are not adjacent to each other. However, assigning structural case to noun phrase by preposition and also assigning structural case to indefinite direct object by verb are subject to adjacency constraints. Therefore, there is a direct relationship between adjacency condition and specificity.
 

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 In the world languages, subject and direct object which are also called grammatical relations are distinguished by three strategies in a sentence: word order, agreement and case marking system. World languages might yield one or two of the so-called strategies or might use all of them for distinguishing grammatical relations. In this article these strategies are examined in Shushtari’s transitive clauses. Therefore a questionnaire consists of 62 sentences was prepared for this purpose and interviews were done with native speakers of Shushtari dialect. Data analysis shows that word order is neither used for distinguishing subject nor for direct object. Agreement is applied for subject and case marking system for direct object in this dialect. As Shushtari is classified in the same dialectic branch with Persian, it is considered to yield the same strategies which are confirmed through data analysis.

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The essay "Discourse Analysis of Imposed War Memories; case study of Da Memory" by Mohammad Reza Javadi Yeganeh and Seyyed Mohammad Ali Sohofi has had the potential for becoming a good and acceptable essay because it adopts a new approach to a controversial text that has bot been analyzed from such viewpoints yet. However, this potential has not been realized. As it is mentioned in the "Introduction" of the essay, the primary objective of the researchers has been to examine the discourse of Da based on Norman Fairclough's model of critical discourse analysis and with regard to the way gender roles are represented in this text. Nevertheless, and this is the weak point of the essay, the authors do not fulfill what they promise at the beginning of their writing, and the result is not "critical discourse analysis" but a simple analysis that at best appears silimilar to Feminist or gender criticism.

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The purpose of this research is to analyze Differential Adpositional Case Marking (DACM) in Vafsi within OT framework by using the generalization of Aissen’s (2003) Differential Object Marking (DOM) model. While Bossong (1985) has traced DOM in more than 300 languages, DACM has not been investigated typologically, yet. DACM, as a branch of Differential Case Marking (DCM), questions the effect of semantic and/or pragmatic features on case marking of object of adposition. Evidence from Vafsi (Stilo, 2004) suggest that animacy in object of adposition (OA), affects its case marking; i. e. human and animate OAs in Vafsi will be oblique case-marked, while inanimate OAs are direct (nominative) case-marked. DACM OT model is developed using Prince and Smolensky (1993), Smolensky (1995) and the generalization of Aissen’s (2003) DOM OT model. The constraints hierarchies are extracted using formal devices of harmonic alignment and local conjunction.

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Recently, many studies have been conducted to produce lactic acid. At present study, lactic acid production conditions from whey, in batch fermentation, by Lactobacillus casei were studied to optimize culture medium, using Taguchi method. Four different concentrations for nitrogen sources; yeast extract, peptone and ammonium sulfate; and 3 levels for temperature and shaker rate were selected. At predicted optimum conditions by Taguchi analysis, in batch fermentation and without pH control, after 24 hrs incubation, 12.5 g/lit lactic acid was obtained.  

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Mastitis deteriorates the quality of raw milk and higher proteolytic activity in mastitic milk has been reported. There are no published studies on the effects of health status of the animal on the casein micelle microstructure, therefore the specific objective of this study was to determine the effects of somatic cell count (SCC) on the dimension and microstructure of casein micelles in raw milk. Raw milk samples were collected from the individual quarter of the dairy cattle in three different levels of SCCs (i.e., low: SCC<200,000, medium: 200,000<SCC<800,000 and high: SCC >800,000 cells/ml) and prepared for study by Scanning Electron Microscopy (SEM). The results suggested that the size of casein micelle changes in mastitic milk, and there were significant differences in casein micelle microstructure between high and low SCC milk samples. The mean diameter of micelles in medium and high SCC specimen decreases dramatically and SEM micrographs revealed that aggregation of casein micelles increases considerably in raw milk samples, especially in high SCC milk, except for that of low SCC. Increase the activity of proteolytic enzymes such as plasmin and lysosomal enzymes (Elastase, Cathepsin B, D and G, etc) was realized as the main reason of hydrolyzing casein in milk, especially in milk with high SCC, which can be the cause of changes in size and tendency to aggregation of casein micelles in mastitic milk. In addition, decreased milk synthesis ability and reduced steric and electrostatic repulsion among casein micelles might be other causes for this phenomenon.  

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Uricase or urate oxidase is an enzyme that converts uric acid (with low solubility) to 5-hydroxyisourate and finally to allantoin. The possibility of developing some diseases like gout and kidney stones will be increased in high levels of uric acid. Thus, uricase can be used as drug enzyme to reduce uric acid levels in the blood. The low stability of proteins (such as drug enzymes) is a challenge in the use of them. There are several approaches such as use of additives for protein stabilization. In this study, E. Coli BL21 (DE3) was transformed by pET28a (+) vector carrying Aspergillus flavus uricase gene. The recombinant protein was expressed and then purified by a Ni-NTA agarose chromatography column. After purification, the thermal stability of the purified enzyme was evaluated and then it stabilized by additives. The results showed that enzyme is active and purified very well. Thermal stability results indicated that uricase maintains its stability up to 20°C and then loses its stability. The half-life of enzyme was 30minutes at 40 °. The results of enzyme stabilization by 20% (v/w) concentration of glucose and sorbitol as well as by 20 % (v/v) of glycerol showed that glucose had the most stabilization effect on the uricase among the additives. The stability (half life) of enzyme was increased more than two times in the presence of glucose. Finally, we conclude that additives like glucose which increase surface tension have the most stabilization effect on the uricase enzyme stability.

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Aim and Background: Amylloid fibrils are filamentus protein aggregates derived from various proteins and peptides. They can be distinguished from other type of features according to their appear shape and electron microscope images, also by dye binding methods, which can indicate induced cross beta structures. Amyloid fibrils are correlated to creating general disease, amyloidosis. Disease such Alzheimer, Parkinson, diabetes type II, and others disease which in each of them, the special kind of protein subjected to form amyloid or amyloid like fibrils. A variety of proteins which they are not converted to amyloid fibrils invivo, can be transform to amyloids in special unstabilizing conditions.
Materials and Methods: Congored spectrophotometric method, ThT fluorescence and CD Data was used for fibril formation assay and Transmission Electron microscopy was used for final affirmation of fibrils.
Results: results shows that maximum amyloid formation was in 5 mg.ml-1 protein concentration, 50 ºC and 7.4 buffer pH.

Conclusion:
With the new approach obtained from the kappa casein, amyloid fibers can be introduced as new nanomaterials, Thus the results, given the diverse applications of nanomaterials, can affirm process optimization of amyloid production from accessible and inexpensive protein in milk.